Analytical Data
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Gene name
UGT71C1
- Application
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Alternative Names
UGT71C1；Flavonol 3-O-glucosyltransferase UGT71C1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O82381
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Expression Region
1-481aa
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AA Sequence
MGKQEDAELVIIPFPFSGHILATIELAKRLISQDNPRIHTITILYWGLPFIPQADTIAFLRSLVKNEPRIRLVTLPEVQDPPPMELFVEFAESYILEYVKKMVPIIREALSTLLSSRDESGSVRVAGLVLDFFCVPMIDVGNEFNLPSYIFLTCSAGFLGMMKYLPERHREIKSEFNRSFNEELNLIPGYVNSVPTKVLPSGLFMKETYEPWVELAERFPEAKGILVNSYTALEPNGFKYFDRCPDNYPTIYPIGPILCSNDRPNLDSSERDRIITWLDDQPESSVVFLCFGSLKNLSATQINEIAQALEIVDCKFIWSFRTNPKEYASPYEALPHGFMDRVMDQGIVCGWAPQVEILAHKAVGGFVSHCGWNSILESLGFGVPIATWPMYAEQQLNAFTMVKELGLALEMRLDYVSEDGDIVKADEIAGTVRSLMDGVDVPKSKVKEIAEAGKEAVDGGSSFLAVKRFIGDLIDGVSISK
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Molecular Weight
56.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UGT71C1 is a UDP-glucuronosyltransferase, which plays a vital role in the glucuronidation process, a key phase II metabolic pathway that conjugates various endogenous and exogenous compounds to facilitate their excretion. This enzyme is particularly significant in plants, as it contributes to the detoxification of secondary metabolites and environmental pollutants. The study of UGT71C1 and its recombinant protein expression has garnered interest due to its potential applications in biotechnology and pharmacology. By understanding the enzyme's substrate specificity, kinetic properties, and structure-function relationships, researchers aim to enhance its efficiency for industrial applications, such as bioremediation and the production of pharmacologically active compounds. Furthermore, elucidating the mechanisms behind its activity could provide insights into metabolic engineering for improving plant resistance to stressors and enhancing the production of valuable metabolites. Recent advancements in molecular biology techniques have enabled the successful expression and characterization of UGT71C1, paving the way for future studies that could leverage its capabilities in various biotechnological innovations.
