Analytical Data
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Gene name
RSAFD1
- Application
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Alternative Names
TYW1; RSAFD1; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1; EC 4.1.3.44; Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; tRNA wybutosine-synthesizing protein 1 homolog; tRNA-yW-synthesizing protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NV66
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Expression Region
1-732 aa
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AA Sequence
MDPSADTWDL FSPLISLWIN RFYIYLGFAV SISLWICVQI VIKTQGKNLQ EKSVPKAAQD LMTNGYVSLQ EKDIFVSGVK IFYGSQTGTA KGFATVLAEA VTSLDLPVAI INLKEYDPDD HLIEEVTSKN VCVFLVATYT DGLPTESAEW FCKWLEEASI DFRFGKTYLK GMRYAVFGLG NSAYASHFNK VGKNVDKWLW MLGAHRVMSR GEGDCDVVKS KHGSIEADFR AWKTKFISQL QALQKGERKK SCGGHCKKGK CESHQHGSEE REEGSHEQDE LHHRDTEEEE PFESSSEEEF GGEDHQSLNS IVDVEDLGKI MDHVKKEKRE KEQQEEKSGL FRNMGRNEDG ERRAMITPAL REALTKQGYQ LIGSHSGVKL CRWTKSMLRG RGGCYKHTFY GIESHRCMET TPSLACANKC VFCWRHHTNP VGTEWRWKMD QPEMILKEAI ENHQNMIKQF KGVPGVKAER FEEGMTVKHC ALSLVGEPIM YPEINRFLKL LHQCKISSFL VTNAQFPAEI RNLEPVTQLY VSVDASTKDS LKKIDRPLFK DFWQRFLDSL KALAVKQQRT VYRLTLVKAW NVDELQAYAQ LVSLGNPDFI EVKGVTYCGE SSASSLTMAH VPWHEEVVQF VHELVDLIPE YEIACEHEHS NCLLIAHRKF KIGGEWWTWI DYNRFQELIQ EYEDSGGSKT FSAKDYMART PHWALFGASE RGFDPKDTRH QRKNKSKAIS GC
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Molecular Weight
83.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the recombinant protein RSAFD1 has garnered significant interest due to its potential roles in plant stress responses and development. RSAFD1, a member of the Arabidopsis thaliana family of proteins, is believed to be involved in the modulation of abiotic stress tolerance, particularly under conditions of drought and salinity. Research has shown that RSAFD1 expression is upregulated in response to various environmental stresses, suggesting its crucial role in plant adaptation mechanisms. Understanding the molecular function of RSAFD1 can provide insights into the intricate signaling pathways that confer stress resilience in plants. Moreover, the exploration of RSAFD1 as a recombinant protein could lead to innovative biotechnological applications aimed at improving crop yield and sustainability in the face of climate change challenges. By utilizing advanced techniques in molecular biology and protein engineering, researchers aim to elucidate the structural and functional characteristics of RSAFD1, paving the way for its application in genetic engineering for enhanced stress resistance in agricultural species. As global food security becomes increasingly threatened by environmental changes, the investigation of RSAFD1 and its interactions at the cellular level could not only contribute to fundamental plant biology but also hold significant implications for agricultural practices.
