Analytical Data
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基因名
uxuA
- Application
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别名
uxuA；Mannonate dehydratase
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24215
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表达区间
1-394aa
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氨基酸序列
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR
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分子量
44.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of uxuA recombinant protein is rooted in the exploration of bacterial metabolic pathways, specifically those involving the catabolism of complex carbohydrates. The uxuA gene encodes for a key enzyme involved in the degradation of ascorbate and other polyol compounds, playing a critical role in the utilization of certain sugars by bacteria, particularly in environments rich in plant materials. As researchers aim to understand the functional dynamics of this protein, its implications for metabolic engineering, environmental microbiology, and potential biotechnological applications are becoming increasingly evident. Recombinant proteins like uxuA offer insights into enzymatic mechanisms, substrate specificity, and the regulatory roles of such enzymes in microbial ecosystems. Furthermore, the ability to produce and study the uxuA protein in vitro provides valuable opportunities to investigate its kinetic properties and stability under various conditions. This research not only contributes to the fundamental understanding of microbial metabolism but also holds promise for applications in waste management, biofuel production, and the development of novel biocatalysts for industrial processes. Overall, the uxuA recombinant protein serves as a model for future studies aimed at harnessing microbial systems for sustainable biotechnological advancements.
