Analytical Data
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Gene name
CDC40
- Application
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Alternative Names
Cdc40; Cell division cycle 40; Cell division cycle 40 homolog; EH binding Protein 3; EH-binding Protein 3; Ehb3; hPRP17
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60508
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Expression Region
1-579aa
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AA Sequence
MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD
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Molecular Weight
91.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CDC40, also known as pre-mRNA-splicing factor Cdc40, is a crucial component of the spliceosome, a molecular machine responsible for the removal of introns from precursor mRNA in eukaryotic cells. The proper functioning of CDC40 is essential for gene expression regulation and the generation of mature mRNA transcripts. Mutations or dysregulation of CDC40 have been linked to various diseases, including cancer, highlighting its importance in cellular function. Recent research has focused on the structural and functional characterization of CDC40 to better understand its role in the splicing process and its interactions with other spliceosomal components. Recombinant CDC40 proteins are often produced for biochemical studies, allowing for in-depth analysis of its activity and interactions in vitro. This research is aimed at elucidating the mechanistic details of splicing and may provide insights that could lead to the development of novel therapeutic strategies for diseases associated with splicing defects. Understanding CDC40's role in the spliceosome not only enhances our grasp of fundamental cellular processes but also holds potential for translational applications in medicine.











