Cat: PA2000-2868

Recombinant E.coli cry1Ab Protein,His

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Analytical Data

  • 基因名

    cry1Ab

  • Application

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    cry1Ab;Cryptochrome-1

  • 种属

    E.coli

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P0A370

  • 表达区间

    1022-1155aa

  • 氨基酸序列

    HEIENNTDELKFSNCVEEEVYPNNTVTCNDYTATQEEYEGTYTSRNRGYDGAYESNSSVPADYASAYEEKAYTDGRRDNPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE

  • 分子量

    17.3 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

Cry1Ab recombinant protein is derived from the bacterium *Bacillus thuringiensis* (Bt), known for its insecticidal properties. This protein has garnered significant interest in agricultural biotechnology due to its effectiveness against various insect pests, particularly from the orders Lepidoptera, which includes many harmful agricultural pests. The research into Cry1Ab began in the late 20th century when scientists discovered that Bt produces proteins that are toxic to specific insects upon ingestion. These proteins act by binding to midgut receptors in the insects, leading to cell lysis and ultimately death. The ability to express Cry1Ab in genetically modified crops, such as Bt cotton and Bt corn, provides a sustainable solution for pest control, reducing the reliance on chemical pesticides and promoting environmentally friendly farming practices. Studies have focused on optimizing the expression levels, stability, and efficacy of the Cry1Ab protein to enhance its pest resistance properties. Furthermore, understanding the molecular mechanisms of its action and potential non-target effects is crucial for evaluating the safety and ecological impact of Bt crops. As agricultural demands grow alongside concerns about insect resistance and environmental sustainability, the research on Cry1Ab recombinant protein continues to play a pivotal role in developing innovative pest management strategies.

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IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
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