Analytical Data
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基因名
ptsH
- Application
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别名
ptsH;Phosphocarrier Protein HPr
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种属
Bacillus subtilis
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08877
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表达区间
2-88aa
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氨基酸序列
AQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSEGLGE
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分子量
25.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of PtsH recombinant protein is rooted in its critical role in cellular signaling and metabolic regulation in various organisms. PtsH, part of the phosphotransferase system (PTS), is involved in the transport and phosphorylation of carbohydrates, influencing energy production and glucose metabolism. Research has shown that PtsH acts as a signaling molecule, thereby modulating the activity of downstream pathways in response to nutrient availability. Its function is not limited to carbohydrate metabolism; it also plays a role in stress response and adaptation by influencing gene expression and protein interactions. Investigating PtsH at the molecular level through recombinant protein technologies enables researchers to isolate and characterize its specific interactions, understand its structural properties, and assess its functional implications in cellular processes. This has significant biotechnological potential, particularly in the development of microbial strains for industrial fermentation processes, where optimized carbohydrate metabolism can enhance productivity. Furthermore, studies on recombinant PtsH contribute to a broader understanding of bacterial physiology and can inform therapeutic strategies, especially in the context of pathogenic organisms that utilize similar metabolic pathways for survival and virulence. Thus, the investigation of PtsH and its recombinant forms provides valuable insights into fundamental biological processes and offers opportunities for applicable advancements in biotechnology and medicine.












