Analytical Data
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基因名
RIC8A
- Application
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别名
MGC104517; MGC131931; MGC148073; MGC148074; Protein Ric-8A; resistance to inhibitors of cholinesterase 8 homolog A (C. elegans); RIC8; RIC8A; RIC8A_HUMAN; Synembryn-A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NPQ8
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表达区间
1-531 aa
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氨基酸序列
MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL VSVLEQGLPP SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV SEGSVPESAD MDVVLESLKC LCNLVLSSPV AQMLAAEARL VVKLTERVGL YRERSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELKGVR LLTDTLELTL GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV DEEDAALYRH LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK FLKAQVLPPL RDVRTRPEVG EMLRNKLVRL MTHLDTDVKR VAAEFLFVLC SESVPRFIKY TGYGNAAGLL AARGLMAGGR PEGQYSEDED TDTDEYKEAK ASINPVTGRV EEKPPNPMEG MTEEQKEHEA MKLVTMFDKL SRNRVIQPMG MSPRGHLTSL QDAMCETMEQ QLSSDPDSDP D
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分子量
59.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of RIC8A, a guanine nucleotide exchange factor (GEF) for the heterotrimeric G protein subunits, has gained significant interest due to its pivotal role in cellular signaling pathways. Heterotrimeric G proteins are crucial mediators of signal transduction in various physiological processes, including hormone signaling, sensory perception, and immune responses. RIC8A, by facilitating the exchange of GDP for GTP on Gα subunits, activates these proteins, thus influencing downstream signaling cascades. Recent research has uncovered the importance of RIC8A in regulating neuronal development and function, as well as its involvement in the pathology of several diseases, such as cancer and neurodegenerative disorders. Understanding the structural and functional properties of RIC8A, as well as its interactions with G proteins and other cellular components, is essential for elucidating its biological significance. Recombinant RIC8A proteins have been produced to study their biochemical properties and to explore their potential as therapeutic targets. This research aims to provide insights into the molecular mechanisms of G protein signaling and to identify new strategies for intervention in disease states linked to dysregulated G protein activity.
