Analytical Data
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Gene name
CA12
- Application
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Alternative Names
CA12；Collagen alpha-1(II) chain
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43570
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Expression Region
25-301aa
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AA Sequence
APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
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Molecular Weight
33.1kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CA12, or Carbonic Anhydrase 12, is an enzyme that plays a crucial role in regulating carbon dioxide and bicarbonate levels in various biological systems. It belongs to the α-carbonic anhydrase family and is primarily expressed in human kidney and other tissues. The study of CA12 has garnered attention due to its potential implications in various physiological and pathological processes, including renal function, acid-base balance, and tumor progression. Researchers have identified CA12's involvement in cancer biology, particularly its overexpression in certain tumors, which may contribute to the acidic microenvironment that supports tumor growth and metastasis. Understanding the structural characteristics of CA12 through recombinant protein studies has enabled scientists to explore its enzymatic mechanisms, inhibitor design, and potential therapeutic applications. Moreover, the development of CA12-specific inhibitors may offer innovative strategies for cancer treatment by targeting the pH regulation within tumors. Overall, research on CA12 is paving the way for novel insights into its biological functions and therapeutic potential, making it a significant focus in biochemistry and medical research.
