Analytical Data
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基因名
phoP
- Application
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别名
phoP；Phosphoethanolamine/phosphocholine phosphatase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23836
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表达区间
1-223aa
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氨基酸序列
MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHIPDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFELR
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分子量
29.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PhoP is a key regulatory protein involved in the phosphate response signaling pathway in various bacteria, particularly in the model organism Escherichia coli. This protein plays a crucial role in enabling bacteria to adapt to low phosphate conditions by regulating the expression of genes responsible for phosphate acquisition and utilization. The study of PhoP and its recombinant protein form is significant for understanding the mechanisms underlying bacterial adaptation and survival in nutrient-limited environments. Recent research has highlighted PhoP's role not only in phosphate regulation but also in its interactions with other regulatory networks, influencing virulence factors and biofilm formation in pathogens. By utilizing recombinant DNA technology to produce PhoP protein, researchers aim to unravel its structure-function relationships and post-translational modifications. This knowledge can facilitate the development of novel antibacterial strategies, especially considering the rising challenge of antibiotic resistance. Understanding PhoP's regulatory mechanisms can also inspire biotechnological applications, such as optimizing microbial strains for bioremediation or biofertilization. Overall, investigating recombinant PhoP protein offers valuable insights into bacterial physiology and potential applications in health and environmental sustainability.
