Analytical Data
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Gene name
cry2Ab
- Application
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Alternative Names
cry2Ab；cryB2；cryIIA(b)；Pesticidal crystal Protein Cry2Ab
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21254
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Expression Region
1-633aa
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AA Sequence
MNSVLNSGRTTICDAYNVAAHDPFSFQHKSLDTVQKEWTEWKKNNHSLYLDPIVGTVASFLLKKVGSLVGKRILSELRNLIFPSGSTNLMQDILRETEKFLNQRLNTDTLARVNAELTGLQANVEEFNRQVDNFLNPNRNAVPLSITSSVNTMQQLFLNRLPQFQMQGYQLLLLPLFAQAANLHLSFIRDVILNADEWGISAATLRTYRDYLKNYTRDYSNYCINTYQSAFKGLNTRLHDMLEFRTYMFLNVFEYVSIWSLFKYQSLLVSSGANLYASGSGPQQTQSFTSQDWPFLYSLFQVNSNYVLNGFSGARLSNTFPNIVGLPGSTTTHALLAARVNYSGGISSGDIGASPFNQNFNCSTFLPPLLTPFVRSWLDSGSDREGVATVTNWQTESFETTLGLRSGAFTARGNSNYFPDYFIRNISGVPLVVRNEDLRRPLHYNEIRNIASPSGTPGGARAYMVSVHNRKNNIHAVHENGSMIHLAPNDYTGFTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQNNTTARYTLRGNGNSYNLYLRVSSIGNSTIRVTINGRVYTATNVNTTTNNDGVNDNGARFSDINIGNVVASSNSDVPLDINVTLNSGTQFDLMNIMLVPTNISPLY
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Molecular Weight
74.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cry2Ab is a recombinant protein derived from the bacterium *Bacillus thuringiensis* (Bt), which is renowned for its insecticidal properties against a variety of agricultural pests. The research background of Cry2Ab primarily revolves around its potential application in biopesticides and genetically modified crops aimed at pest management. As global agricultural practices face increasing challenges from pest resistance to conventional chemical pesticides, Cry2Ab offers a sustainable alternative due to its specificity and ability to target only certain insect species, thereby minimizing harm to non-target organisms and preserving ecological balance. Studies have demonstrated that Cry2Ab possesses a significant mode of action, disrupting the gut cells of susceptible insects upon ingestion, leading to their mortality while being safe for humans and beneficial insects. The recombinant expression of Cry2Ab in plant systems or as a standalone biopesticide has garnered attention for its potential to enhance crop yield and reduce reliance on synthetic insecticides. Furthermore, understanding the molecular mechanisms underlying its activity and stability can pave the way for the development of more effective pest-resistant plant varieties. The ongoing research aims to optimize production processes, assess environmental impact, and evaluate the economic feasibility of deploying Cry2Ab in agricultural settings. As such, the exploration of Cry2Ab continues to be a pivotal area of study in the quest for innovative solutions to enhance global food security while promoting sustainable agricultural practices.
