Analytical Data
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基因名
gadA
- Application
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别名
gadA；Glutamate decarboxylase A
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P69908
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表达区间
1-466aa
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氨基酸序列
MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
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分子量
56.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of GadA, a glutamate decarboxylase enzyme, has gained significant attention due to its pivotal role in the acid tolerance response of various microorganisms, particularly in foodborne pathogens like Escherichia coli. GadA catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), a process that not only helps in maintaining pH homeostasis under acidic conditions but also contributes to microbial survival and virulence. Recombinant GadA proteins have been expressed in heterologous systems to facilitate detailed investigations into their enzymatic properties, structural characteristics, and functional mechanisms. These studies are crucial for understanding the adaptive strategies of bacteria in acidic environments, as well as for potential applications in food safety and biotechnology. Furthermore, the insights gained from GadA research can inform the development of novel antimicrobial strategies and the enhancement of probiotic formulations, thereby leveraging its biological functions for health benefits. Ongoing research aims to elucidate the regulation of GadA expression, its interaction with other cellular pathways, and its effect in different microbial species, highlighting its significance in both basic and applied microbiology.
