Analytical Data
-
Gene name
RAD21
- Application
-
Alternative Names
CDLS4; Double-strand-break repair protein rad21 homolog; hHR21; HR21; HRAD21; KIAA0078; MCD1; Nuclear matrix protein 1; NXP-1; NXP1; Protein involved in DNA double-strand break repair; RAD21; RAD21 homolog (S. pombe); RAD21 homolog; RAD21_HUMAN; Scc1; SCC1 homolog
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O60216
-
Expression Region
1-631 aa
-
AA Sequence
MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN REAAYNAITL PEEFHDFDQP LPDLDDIDVA QQFSLNQSRV EEITMREEVG NISILQENDF GDFGMDDREI MREGSAFEDD DMLVSTTTSN LLLESEQSTS NLNEKINHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG IFDDPPALSE AGVMLPEQPA HDDMDEDDNV SMGGPDSPDS VDPVEPMPTM TDQTTLVPNE EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL MMWKETGGVE KLFSLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF ENPEVPREDQ QQQHQQRDVI DEPIIEEPSR LQESVMEASR TNIDESAMPP PPPQGVKRKA GQIDPEPVMP PQQVEQMEIP PVELPPEEPP NICQLIPELE LLPEKEKEKE KEKEDDEEEE DEDASGGDQD QEERRWNKRT QQMLHGLQRA LAKTGAESIS LLELCRNTNR KQAAAKFYSF LVLKKQQAIE LTQEEPYSDI IATPGPRFHI I
-
Molecular Weight
71.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RAD21 is a critical component of the cohesion complex, playing a vital role in the maintenance of chromosomal stability during cell division. This protein is essential for sister chromatid cohesion, which ensures proper chromosome segregation during mitosis and meiosis. Dysregulation or mutations in RAD21 have been implicated in various cancers, highlighting its importance in genomic integrity. The study of RAD21 recombinant protein has garnered attention as it provides insight into the mechanisms of chromosomal dynamics, DNA repair, and the cellular response to DNA damage. Researchers often produce RAD21 in recombinant systems to study its structure, interaction with other proteins, and regulatory pathways. Understanding RAD21’s function and interactions can reveal novel therapeutic targets for cancer treatment and improve our understanding of chromosomal behavior in health and disease. The continued investigation of RAD21 and its recombinant forms is crucial for unraveling the complexities of cell cycle regulation and associated disorders.
