Analytical Data
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Gene name
PUSL1
- Application
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Alternative Names
PUSL1; tRNA pseudouridine synthase-like 1; EC 5.4.99.-; tRNA pseudouridylate synthase-like 1; tRNA-uridine isomerase-like 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N0Z8
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Expression Region
1-303 aa
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AA Sequence
MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG SHG
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Molecular Weight
33.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PUSL1, or Pseudouridine Synthase and Leucine-rich Repeat Protein 1, has emerged as a significant research target due to its role in RNA modification and cellular function. Pseudouridine is a modified nucleoside found in various RNA molecules, and its synthesis is facilitated by enzymes known as pseudouridine synthases. PUSL1 belongs to this family and is thought to play a critical role in post-transcriptional RNA processing, influencing mRNA stability, translation efficiency, and the overall dynamics of gene expression. Increased evidence suggests that PUSL1 may be linked to various biological processes, including stress response, cell differentiation, and proliferation. Moreover, alterations in its expression or function have been associated with several diseases, including cancer and neurological disorders. Investigating PUSL1 could provide insights into its mechanistic roles in RNA biology and potential therapeutic targets for diseases resulting from dysregulated RNA modifications. This growing interest underscores the necessity for rigorous studies to elucidate PUSL1’s structure, enzymatic activity, and broader implications in cellular homeostasis and pathology.
