Analytical Data
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基因名
PRPF18
- Application
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别名
PRPF18; HPRP18Pre-mRNA-splicing factor 18; PRP18 homolog; hPRP18
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99633
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表达区间
1-342 aa
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氨基酸序列
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
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分子量
39.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRPF18 is a crucial protein involved in the splicing of pre-mRNA, playing a vital role in gene expression regulation. It is an essential component of the spliceosome, a complex responsible for the removal of introns from pre-mRNA transcripts. Mutations in the PRPF18 gene have been associated with various diseases, particularly retinitis pigmentosa, a progressive retinal degenerative disorder that leads to vision loss. Understanding the structure and function of PRPF18 is paramount for elucidating its role in splicing and investigating its implications in disease mechanisms. Recombinant PRPF18 proteins are being studied to gain insights into the protein's biochemical properties, interactions with other spliceosomal components, and the impact of disease-related mutations. These studies employ techniques such as protein expression and purification, biochemical assays, and structural analysis to explore the functional dynamics of PRPF18. By elucidating the molecular basis of its action, researchers aim to develop potential therapeutic strategies for disorders linked to PRPF18 dysfunction. This avenue of research highlights the significance of basic science in addressing complex genetic conditions and paves the way for future innovations in gene therapy and genetic medicine.
