Analytical Data
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Gene name
PRPF18
- Application
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Alternative Names
PRPF18; HPRP18Pre-mRNA-splicing factor 18; PRP18 homolog; hPRP18
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99633
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Expression Region
1-342 aa
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AA Sequence
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
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Molecular Weight
39.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRPF18 is a crucial protein involved in the splicing of pre-mRNA, playing a vital role in gene expression regulation. It is an essential component of the spliceosome, a complex responsible for the removal of introns from pre-mRNA transcripts. Mutations in the PRPF18 gene have been associated with various diseases, particularly retinitis pigmentosa, a progressive retinal degenerative disorder that leads to vision loss. Understanding the structure and function of PRPF18 is paramount for elucidating its role in splicing and investigating its implications in disease mechanisms. Recombinant PRPF18 proteins are being studied to gain insights into the protein's biochemical properties, interactions with other spliceosomal components, and the impact of disease-related mutations. These studies employ techniques such as protein expression and purification, biochemical assays, and structural analysis to explore the functional dynamics of PRPF18. By elucidating the molecular basis of its action, researchers aim to develop potential therapeutic strategies for disorders linked to PRPF18 dysfunction. This avenue of research highlights the significance of basic science in addressing complex genetic conditions and paves the way for future innovations in gene therapy and genetic medicine.
