Analytical Data
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Gene name
ACAT2
- Application
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Alternative Names
ACAT2；ACTL；Acetyl-CoA acetyltransferase. cytosolic
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BWD1
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Expression Region
1-397aa
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AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTDSILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE
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Molecular Weight
45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACAT2 (Acyl-CoA:cholesterol acyltransferase 2) is an enzyme that plays a crucial role in the intracellular esterification of cholesterol, linking lipid metabolism to cellular processes and disease states. Research into ACAT2 gained momentum due to its association with various metabolic disorders, including atherosclerosis and non-alcoholic fatty liver disease, making it a potential therapeutic target. Unlike its counterpart ACAT1, ACAT2 is predominantly expressed in the intestines and is responsible for the absorption of dietary cholesterol. Understanding the regulation and function of ACAT2 is critical as it may influence cholesterol homeostasis and lipid storage, which are key factors in cardiovascular health. Furthermore, recent studies have highlighted the importance of ACAT2 in inflammation and the immune response, suggesting a complex role in both metabolic and inflammatory pathways. The development of recombinant ACAT2 proteins allows for detailed structural and functional studies, facilitating the exploration of enzymatic mechanisms and the discovery of inhibitors that could lead to innovative treatments for related diseases. Thus, the investigation of ACAT2 recombinant proteins holds promise for advancing our understanding of lipid biology and developing targeted interventions for cholesterol-related health issues.
