Analytical Data
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Gene name
DNM1L
- Application
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Alternative Names
DNM1L；DLP1；DRP1；Dynamin-1-like Protein
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Species
Human
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Source
E. coli
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Tag
N-terminal His Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00429-3
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Expression Region
1-710aa
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AA Sequence
MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
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Molecular Weight
82.8kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
Dynamin-related protein 1-like (DNM1L), also known as Drp1, is a member of the dynamin family of large GTPases, playing a crucial role in mitochondrial dynamics, particularly in mitochondrial fission. In recent years, research has increasingly focused on DNM1L due to its involvement in various cellular processes, including apoptosis, autophagy, and the maintenance of mitochondrial integrity. Abnormalities in DNM1L function have been implicated in several diseases, such as neurodegenerative disorders, metabolic syndromes, and cancer, highlighting its potential as a therapeutic target. The recombinant expression of DNM1L allows for detailed studies on its structure-function relationship and its regulatory mechanisms. By producing and purifying this protein in a laboratory setting, researchers can investigate its GTPase activity, interactions with other mitochondrial proteins, and role in cellular signaling pathways. Cloning and expression systems, such as bacterial or eukaryotic systems, are commonly used to generate active DNM1L, enabling functional assays and high-resolution structural studies. Understanding DNM1L's molecular mechanisms can provide valuable insights into mitochondrial biology and disease pathogenesis, positioning DNM1L as a key player in the fields of cell biology and therapeutic development. This burgeoning interest underlines the imperative of continued research into DNM1L and its recombinant protein forms to unravel its multifaceted roles in health and disease.
