Analytical Data
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Gene name
POLA2
- Application
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Alternative Names
AI573378; DNA directed DNA polymerase alpha 2; DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha subunit B; DNA polymerase alpha subunit II; DNA polymerase subunit alpha B; DNA polymerase subunit II; DPOA2_HUMAN; FLJ21662; FLJ37250; polA2; Polymerase (DNA directed) alpha 2 (70kD subunit); Polymerase (DNA directed); alpha (70kD); Polymerase (DNA directed); alpha 2 accessory subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14181
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Expression Region
1-598 aa
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AA Sequence
MSASAQQLAE ELQIFGLDCE EALIEKLVEL CVQYGQNEEG MVGELIAFCT STHKVGLTSE ILNSFEHEFL SKRLSKARHS TCKDSGHAGA RDIVSIQELI EVEEEEEILL NSYTTPSKGS QKRAISTPET PLTKRSVSTR SPHQLLSPSS FSPSATPSQK YNSRSNRGEV VTSFGLAQGV SWSGRGGAGN ISLKVLGCPE ALTGSYKSMF QKLPDIREVL TCKIEELGSE LKEHYKIEAF TPLLAPAQEP VTLLGQIGCD SNGKLNNKSV ILEGDREHSS GAQIPVDLSE LKEYSLFPGQ VVIMEGINTT GRKLVATKLY EGVPLPFYQP TEEDADFEQS MVLVACGPYT TSDSITYDPL LDLIAVINHD RPDVCILFGP FLDAKHEQVE NCLLTSPFED IFKQCLRTII EGTRSSGSHL VFVPSLRDVH HEPVYPQPPF SYSDLSREDK KQVQFVSEPC SLSINGVIFG LTSTDLLFHL GAEEISSSSG TSDRFSRILK HILTQRSYYP LYPPQEDMAI DYESFYVYAQ LPVTPDVLII PSELRYFVKD VLGCVCVNPG RLTKGQVGGT FARLYLRRPA ADGAERQSPC IAVQVVRI
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Molecular Weight
65.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLA2, a crucial component of the DNA polymerase alpha (pol α) complex, plays a significant role in DNA replication and repair. This enzyme complex is vital for synthesizing the RNA-DNA primer required for initiating DNA replication, making POLA2 essential for maintaining genomic stability. Studies have indicated that POLA2 not only contributes to the polymerase activity but also possesses regulatory roles in the cell cycle and is involved in the coordination of replication stress responses. Given its vital functions, the dysregulation of POLA2 has been associated with various cancers and genetic disorders, highlighting its potential as a therapeutic target. Recent research has focused on elucidating the structural and functional mechanisms of POLA2, including its interactions with other proteins involved in DNA metabolism. Understanding these interactions is crucial for developing targeted therapies that can modulate pol α activity in pathological conditions. The study of POLA2 recombinant protein is also important for further insights into DNA replication mechanisms and the development of molecular tools for gene editing and therapeutic applications.
