Analytical Data
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Gene name
PJA2
- Application
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Alternative Names
E3 ubiquitin protein ligase Praja2; E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap 1; Neurodap1; PJA 2; PJA2; PJA2_HUMAN; Praja 2; Praja 2 RING H2 motif containing; praja ring finger 2; Praja ring finger 2 E3 ubiquitin protein ligase; Praja2; RING finger protein 131; RNF 131; RNF131
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43164
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Expression Region
2-708 aa
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AA Sequence
SQYTEKEPA AMDQESGKAV WPKPAGGYQT ITGRRYGRRH AYVSFKPCMT RHERSLGRAG DDYEVLELDD VPKENSSGSS PLDQVDSSLP SEPIFEKSET EIPTCGSALN QTTESSQSFV AVHHSEEGRD TLGSSTNLHN HSEGEYIPGA CSASSVQNGI ALVHTDSYDP DGKHGEDNDH LQLSAEVVEG SRYQESLGNT VFELENREAE AYTGLSPPVP SFNCEVRDEF EELDSVPLVK SSAGDTEFVH QNSQEIQRSS QDEMVSTKQQ NNTSQERQTE HSPEDAACGP GHICSEQNTN DREKNHGSSP EQVVRPKVRK LISSSQVDQE TGFNRHEAKQ RSVQRWREAL EVEESGSDDL LIKCEEYDGE HDCMFLDPPY SRVITQRETE NNQMTSESGA TAGRQEVDNT FWNGCGDYYQ LYDKDEDSSE CSDGEWSASL PHRFSGTEKD QSSSDESWET LPGKDENEPE LQSDSSGPEE ENQELSLQEG EQTSLEEGEI PWLQYNEVNE SSSDEGNEPA NEFAQPAFML DGNNNLEDDS SVSEDLDVDW SLFDGFADGL GVAEAISYVD PQFLTYMALE ERLAQAMETA LAHLESLAVD VEVANPPASK ESIDGLPETL VLEDHTAIGQ EQCCPICCSE YIKDDIATEL PCHHFFHKPC VSIWLQKSGT CPVCRRHFPP AVIEASAAPS SEPDPDAPPS NDSIAEAP
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Molecular Weight
78.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PJA2 is an E3 ubiquitin ligase that plays a significant role in various cellular processes, including protein degradation, cell signaling, and response to stress. Ubiquitination, a post-translational modification mediated by E3 ligases like PJA2, is crucial for maintaining cellular homeostasis and regulating protein turnover. Research has shown that PJA2 is involved in several important pathways, including those related to cancer progression and immune responses. Given its pivotal function in these biological processes, understanding the structure and functions of PJA2 has become a focus of scientific inquiry. Recent studies have aimed to explore the mechanisms by which PJA2 exerts its effects on cellular functions, including identifying its substrate proteins and downstream signaling pathways. Additionally, the potential of PJA2 as a therapeutic target in various diseases, particularly in oncology, has garnered increasing interest. By investigating PJA2 and its role in the ubiquitin-proteasome system, researchers hope to uncover novel strategies for developing targeted therapies that can modulate its activity and, consequently, address diseases linked to dysregulated protein homeostasis. The ongoing research into recombinant PJA2 proteins aims to further elucidate their functional dynamics and interactions, providing deeper insights into the regulation of cellular processes and potential implications for therapeutic intervention.
