Analytical Data
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Gene name
PIK4CA
- Application
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Alternative Names
EC 2.7.1.67 ; Phosphatidylinositol 4 kinase alpha; Phosphatidylinositol 4 kinase catalytic alpha polypeptide; Phosphatidylinositol 4-kinase alpha; PI4 kinase; PI4-kinase alpha; PI4K ALPHA ; PI4K-alpha; pi4K230; PI4KA; PI4KA_HUMAN; PIK4; PIK4CA; PtdIns 4 kinase; PtdIns-4-kinase alpha
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P42356
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Expression Region
1-854 aa
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AA Sequence
MREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY
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Molecular Weight
123.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIK4CA, a key oncogene, encodes the p110α subunit of phosphoinositide 3-kinase (PI3K), which is pivotal in regulating various cellular processes, including growth, metabolism, and survival. Mutations and aberrant activation of PIK3CA have been implicated in numerous cancers, making it a significant target for therapeutic intervention. Research on PIK4CA recombinant proteins focuses on understanding the structural and functional implications of these mutations, which can drive tumorigenesis. By generating and characterizing PIK4CA recombinant proteins, researchers can facilitate the study of the effects of specific mutations on PI3K activity, downstream signaling pathways, and cellular responses. This knowledge is crucial for the development of targeted therapies aimed at inhibiting mutant forms of the protein, ultimately contributing to more effective cancer treatments. Additionally, PIK4CA recombinant proteins can be used in high-throughput screening assays to identify small molecules that specifically modulate its activity, offering a promising avenue for drug discovery. Overall, the study of PIK4CA recombinant proteins serves as a foundation for advancing our understanding of cancer biology and for the rational design of targeted therapeutics.
