Analytical Data
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基因名
PIGT
- Application
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别名
PIGT; CGI-06; PSEC0163; UNQ716/PRO1379; GPI transamidase component PIG-T; Phosphatidylinositol-glycan biosynthesis class T protein
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q969N2
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表达区间
1-578 aa
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氨基酸序列
MAAAMPLALLVLLLLGPGGWCLAEPPRDSLREELVITPLPSGDVAATFQFRTRWDSELQREGVSHYRLFPKALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWKELSNVLSGIFCASLNFIDSTNTVTPTASFKPLGLANDTDHYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSYHSQAVHIRPVCRNARCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEPCPLASESRVYVDITTYNQDNETLEVHPPPTTTYQDVILGTRKTYAIYDLLDTAMINNSRNLNIQLKWKRPPENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPYRAFPVLLLDTVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQDRLQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSMVAAKPVDWEESPLFNSLFPVSDGSNYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPRTGGLAKRLANLIRRARGVPPL
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分子量
92.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIGT (phosphatidylinositol glycan anchor biosynthesis class T) is an essential enzyme involved in the post-translational modification of proteins through glycosylphosphatidylinositol (GPI) anchoring, a process that plays a crucial role in cell signaling and membrane protein localization. Mutations in the PIGT gene have been associated with various human diseases, including developmental disorders and certain cancers, highlighting its importance in cellular functions and disease mechanisms. Research on PIGT recombinant proteins focuses on elucidating the enzyme's structure-function relationship, the biochemical pathways that lead to GPI biosynthesis, and its interactions with other cellular components. Understanding the PIGT protein's role may provide insights into the pathogenesis of diseases linked to GPI-anchor defects and could lead to potential therapeutic strategies. By harnessing recombinant DNA technology, researchers have been able to produce PIGT proteins in vitro, enabling detailed studies of their enzymatic activities and regulatory mechanisms. As such, PIGT represents a critical target for research aimed at unveiling new avenues for medical intervention in GPI-related diseases.
