Analytical Data
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Gene name
PIGT
- Application
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Alternative Names
PIGT; CGI-06; PSEC0163; UNQ716/PRO1379; GPI transamidase component PIG-T; Phosphatidylinositol-glycan biosynthesis class T protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q969N2
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Expression Region
1-578 aa
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AA Sequence
MAAAMPLALLVLLLLGPGGWCLAEPPRDSLREELVITPLPSGDVAATFQFRTRWDSELQREGVSHYRLFPKALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWKELSNVLSGIFCASLNFIDSTNTVTPTASFKPLGLANDTDHYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSYHSQAVHIRPVCRNARCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEPCPLASESRVYVDITTYNQDNETLEVHPPPTTTYQDVILGTRKTYAIYDLLDTAMINNSRNLNIQLKWKRPPENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPYRAFPVLLLDTVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQDRLQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSMVAAKPVDWEESPLFNSLFPVSDGSNYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPRTGGLAKRLANLIRRARGVPPL
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Molecular Weight
92.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGT (phosphatidylinositol glycan anchor biosynthesis class T) is an essential enzyme involved in the post-translational modification of proteins through glycosylphosphatidylinositol (GPI) anchoring, a process that plays a crucial role in cell signaling and membrane protein localization. Mutations in the PIGT gene have been associated with various human diseases, including developmental disorders and certain cancers, highlighting its importance in cellular functions and disease mechanisms. Research on PIGT recombinant proteins focuses on elucidating the enzyme's structure-function relationship, the biochemical pathways that lead to GPI biosynthesis, and its interactions with other cellular components. Understanding the PIGT protein's role may provide insights into the pathogenesis of diseases linked to GPI-anchor defects and could lead to potential therapeutic strategies. By harnessing recombinant DNA technology, researchers have been able to produce PIGT proteins in vitro, enabling detailed studies of their enzymatic activities and regulatory mechanisms. As such, PIGT represents a critical target for research aimed at unveiling new avenues for medical intervention in GPI-related diseases.
