Analytical Data
-
Gene name
PIGA
- Application
-
Alternative Names
PIGA; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P37287
-
Expression Region
1-169 aa
-
AA Sequence
MAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSIIDVAIDATGPRGAWTNNYSHSKRGGENNEISETR
-
Molecular Weight
45.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGA, or Phosphatidylinositol Glycan Anchor Biosynthesis Class A, is a crucial gene involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are vital for the proper functioning of many proteins on cell surfaces. Research into PIGA and its recombinant protein has garnered attention due to its significant role in cellular processes, including cell signaling, adhesion, and immune response. Mutations in the PIGA gene can lead to various clinical disorders, most notably Paroxysmal Nocturnal Hemoglobinuria (PNH), a rare but serious hematological condition characterized by the destruction of red blood cells. Understanding the structure and function of recombinant PIGA proteins is essential for elucidating their biochemical pathways and could pave the way for novel therapeutic strategies. Additionally, as PIGA is pivotal in the production of GPI-anchored proteins, studying its recombinant form can provide insights into potential applications in gene therapy and cancer treatment, where modulation of GPI-anchored proteins may improve therapeutic outcomes. The exploration of PIGA's recombinant protein is, therefore, a promising area of research that bridges basic science with clinical applications, highlighting its importance in both health and disease management.
