Analytical Data
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Gene name
PDIA5
- Application
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Alternative Names
Pdia5; PDIA5_HUMAN; PDIR; Protein disulfide isomerase related; Protein disulfide isomerase-related protein; Protein disulfide-isomerase A5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14554
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Expression Region
22-519 aa
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AA Sequence
SSAKVSSLI ERISDPKDLK KLLRTRNNVL VLYSKSEVAA ENHLRLLSTV AQAVKGQGTI CWVDCGDAES RKLCKKMKVD LSPKDKKVEL FHYQDGAFHT EYNRAVTFKS IVAFLKDPKG PPLWEEDPGA KDVVHLDSEK DFRRLLKKEE KPLLIMFYAP WCSMCKRMMP HFQKAATQLR GHAVLAGMNV YSSEFENIKE EYSVRGFPTI CYFEKGRFLF QYDNYGSTAE DIVEWLKNPQ PPQPQVPETP WADEGGSVYH LTDEDFDQFV KEHSSVLVMF HAPWCGHCKK MKPEFEKAAE ALHGEADSSG VLAAVDATVN KALAERFHIS EFPTLKYFKN GEKYAVPVLR TKKKFLEWMQ NPEAPPPPEP TWEEQQTSVL HLVGDNFRET LKKKKHTLVM FYAPWCPHCK KVIPHFTATA DAFKDDRKIA CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH YGKFAEKYDS DRTELGFTNY IRALREGDHE RLGKKKEEL
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Molecular Weight
59.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDIA5, or Protein Disulfide Isomerase Family A Member 5, is a member of the protein disulfide isomerase (PDI) family, which plays a crucial role in the formation and rearrangement of disulfide bonds in proteins, essential for proper protein folding and functionality. This protein has garnered attention in various fields, including cancer research, neurodegenerative diseases, and viral infections, due to its involvement in cellular stress responses and its potential as a therapeutic target. Studies have indicated that PDIA5 may influence tumor progression and metastasis by modulating redox homeostasis and protein folding mechanisms within the endoplasmic reticulum (ER). Additionally, its role in the life cycle of certain viruses, such as SARS-CoV-2, highlights the relevance of PDIA5 in understanding viral pathogenesis and developing antiviral strategies. The exploration of PDIA5 functions and mechanisms through recombinant protein studies is essential for elucidating its biological roles and potential implications in disease contexts. By generating and characterizing PDIA5 recombinant proteins, researchers can investigate its structural properties, enzymatic activities, and interactions with other cellular factors, thereby paving the way for novel therapeutic approaches targeting this multifunctional protein. Understanding the nuanced functions of PDIA5 could ultimately contribute to the development of more effective treatments for diseases where protein misfolding and oxidative stress are central pathophysiological features.
