Analytical Data
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Gene name
ARCN1
- Application
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Alternative Names
Archain 1; Archain; Archain vesicle transport Protein 1; Arcn1; Coatomer delta subunit; Coatomer Protein complex
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48444
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Expression Region
1-511aa
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AA Sequence
MVLLAAAVCT KAGKAIVSRQ FVEMTRTRIE GLLAAFPKLM NTGKQHTFVE TESVRYVYQP MEKLYMVLIT TKNSNILEDL ETLRLFSRVI PEYCRALEEN EISEHCFDLI FAFDEIVALG YRENVNLAQI RTFTEMDSHE EKVFRAVRET QEREAKAEMR RKAKELQQAR RDAERQGKKA PGFGGFGSSA VSGGSTAAMI TETIIETDKP KVAPAPARPS GPSKALKLGA KGKEVDNFVD KLKSEGETIM SSSMGKRTSE ATKMHAPPIN MESVHMKIEE KITLTCGRDG GLQNMELHGM IMLRISDDKY GRIRLHVENE DKKGVQLQTH PNVDKKLFTA ESLIGLKNPE KSFPVNSDVG VLKWRLQTTE ESFIPLTINC WPSESGNGCD VNIEYELQED NLELNDVVIT IPLPSGVGAP VIGEIDGEYR HDSRRNTLEW CLPVIDAKNK SGSLEFSIAG QPNDFFPVQV SFVSKKNYCN IQVTKVTQVD GNSPVRFSTE TTFLVDKYEI L
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Molecular Weight
83.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARCN1, also known as Coatomer Protein Complex 1 Subunit Alpha, is a crucial component of the coat protein complex I (COPI), which plays an essential role in the retrograde transport of proteins from the Golgi apparatus back to the endoplasmic reticulum (ER). The study of ARCN1 recombinant protein has garnered significant interest due to its involvement in intracellular trafficking and organelle communication, processes critical for maintaining cellular homeostasis. Disruptions in COPI-mediated transport have been linked to various diseases, including neurodegenerative disorders and cancer, highlighting the importance of understanding ARCN1's function and regulation. Researchers are particularly focused on its structural properties, binding interactions, and the molecular mechanisms underlying its role in vesicular trafficking. By generating recombinant ARCN1, scientists aim to investigate its biochemical characteristics, such as protein-protein interactions and conformational changes upon ligand binding. This research not only enhances our understanding of ARCN1’s specific functions in cellular transport but also opens avenues for potential therapeutic interventions targeting COPI pathways in disease contexts. Ultimately, studying ARCN1 as a recombinant protein could provide valuable insights into the intricate systems of cellular transport and their implications in human health and disease.
