Analytical Data
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Gene name
TCF4
- Application
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Alternative Names
TCF4；BHLHB19；Transcription factor 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P15884
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Expression Region
1-507aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKFKQCR CSDTGLCCLD HEGKAEVYAP SASTADYNRD SPGYPSSKPA TSTFPSSFFM QDGHHSSDPW SSSSGMNQPG YAGMLGNSSH IPQSSSYCSL HPHERLSYPS HSSADINSSL PPMSTFHRSG TNHYSTSSCT PPANGTDSIM ANRGSGAAGS SQTGDALGKA LASIYSPDHT NNSFSSNPST PVGSPPSLSA GTAVWSRNGG QASSSPNYEG PLHSLQSRIE DRLERLDDAI HVLRNHAVGP STAMPGGHGD MHGIIGPSHN GAMGGLGSGY GTGLLSANRH SLMVGTHRED GVALRGSHSL LPNQVPVPQL PVQSATSPDL NPPQDPYRGM PPGLQGQSVS SGSSEIKSDD EGDENLQDTK SSEDKKLDDD KKDIKSITSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDASNHMGQM
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Molecular Weight
57 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TCF4, or Transcription Factor 4, is a member of the β-catenin/T cell factor (TCF) family of transcription factors, predominantly involved in the Wnt signaling pathway, which is crucial for various cellular processes, including cell proliferation, differentiation, and apoptosis. Research on TCF4 has gained significance due to its implications in developmental biology and its association with several psychiatric disorders, notably schizophrenia. The gene encoding TCF4 is located on chromosome 18, and polymorphisms within this gene have been linked to increased risk of psychiatric diseases, suggesting that TCF4 may play a vital role in neurodevelopmental processes. Moreover, TCF4 has been identified as a key regulator in the pathogenesis of various cancers, highlighting its dual role in both normal development and disease states. The study of TCF4 recombinant proteins has emerged as a valuable tool to understand its functional mechanisms, interactions with other proteins, and impact on gene expression. By producing TCF4 in recombinant forms, researchers can explore its binding affinities, post-translational modifications, and regulatory functions in vitro, thereby elucidating its role in cellular signaling pathways. Insights gained from the investigation of TCF4 recombinant proteins not only enhance our understanding of its biological functions but also open avenues for therapeutic interventions in TCF4-related diseases, making it a focal point in current biomedical research.
