Analytical Data
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Gene name
NEK3
- Application
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Alternative Names
NEK3; Serine/threonine-protein kinase Nek3; EC 2.7.11.1; HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51956
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Expression Region
1-506 aa
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AA Sequence
MDDYMVLRMI GEGSFGRALL VQHESSNQMF AMKEIRLPKS FSNTQNSRKE AVLLAKMKHP NIVAFKESFE AEGHLYIVME YCDGGDLMQK IKQQKGKLFP EDMILNWFTQ MCLGVNHIHK KRVLHRDIKS KNIFLTQNGK VKLGDFGSAR LLSNPMAFAC TYVGTPYYVP PEIWENLPYN NKSDIWSLGC ILYELCTLKH PFQANSWKNL ILKVCQGCIS PLPSHYSYEL QFLVKQMFKR NPSHRPSATT LLSRGIVARL VQKCLPPEII MEYGEEVLEE IKNSKHNTPR KKTNPSRIRI ALGNEASTVQ EEEQDRKGSH TDLESINENL VESALRRVNR EEKGNKSVHL RKASSPNLHR RQWEKNVPNT ALTALENASI LTSSLTAEDD RGGSVIKYSK NTTRKQWLKE TPDTLLNILK NADLSLAFQT YTIYRPGSEG FLKGPLSEET EASDSVDGGH DSVILDPERL EPGLDEEDTD FEEEDDNPDW VSELKKRAGW QGLCDR
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Molecular Weight
57.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NEK3 (NIMA-related kinase 3) is a member of the NEK (NIMA-related kinases) family, which plays a crucial role in various cellular processes, including cell cycle regulation, mitosis, and the maintenance of genomic stability. More recently, NEK3 has garnered attention due to its involvement in cancer progression and differentiation. Its overexpression has been linked to tumorigenesis, making it a potential target for therapeutic intervention. Research indicates that NEK3 is not only essential for proper mitotic spindle formation but also influences ciliary function and cellular response to stress. The recombinant expression of NEK3 protein allows for the detailed investigation of its biochemical properties, signaling pathways, and interactions with other cellular components. This has implications for understanding its role in oncogenesis and the potential to develop targeted therapies that could hinder its function in cancer cells. Furthermore, elucidating the structure and functional domains of NEK3 can provide insights into its mechanism of action, leading to innovative strategies in drug development aimed at inhibiting its activity. As a result, the study of NEK3 recombinant protein is vital for advancing our understanding of its biological functions and its potential as a therapeutic target in cancer and other diseases characterized by dysregulated cell division and signaling pathways.
