Analytical Data
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Gene name
RDH12
- Application
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Alternative Names
RDH12;SDR7C2;Retinol dehydrogenase 12
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96NR8
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Expression Region
39-316aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHM SIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS GKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTK DCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE
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Molecular Weight
34 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RDH12 (Retinol Dehydrogenase 12) is an enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family, primarily involved in the metabolism of retinol (vitamin A) and various other substrates. Mutations in the RDH12 gene have been associated with autosomal recessive retinal dystrophies, particularly affecting the photoreceptor cells in the retina, leading to vision impairment and, in severe cases, blindness. Understanding the functional role of RDH12 is crucial not only for elucidating the biochemical pathways of retinol metabolism but also for developing potential therapeutic strategies for retinal diseases linked to its dysfunction. Recent research has focused on the production and characterization of RDH12 recombinant protein to study its enzymatic activity and substrate specificity. By generating RDH12 in different expression systems, researchers can investigate how various factors affect its functionality and stability, ultimately aiming to unravel the underlying mechanisms of retinal degeneration associated with RDH12 mutations. This knowledge can contribute to the design of gene therapy approaches or small molecule interventions that could restore or enhance the enzyme's activity, providing new hope for patients suffering from vision loss due to RDH12-related disorders.
