Analytical Data
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Gene name
NR1D1
- Application
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Alternative Names
NR1D1；EAR1；HREV；THRAL；Nuclear receptor subfamily 1 group D member 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20393
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Expression Region
1-614aa
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AA Sequence
MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPT YFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPP GSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACE GCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSR DAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPM GPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIF TYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR HNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGK APANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREV VEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSR TTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADR SGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNN MHSEKLLSFRVDAQ
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Molecular Weight
93 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NR1D1, also known as Rev-Erbα, is a member of the nuclear hormone receptor superfamily and plays a crucial role in regulating circadian rhythms, metabolism, and inflammation. The study of NR1D1 has gained significant attention due to its involvement in various physiological processes and potential implications in metabolic disorders, such as obesity and diabetes. NR1D1 functions as a transcriptional repressor, regulating the expression of genes involved in the circadian clock and metabolic pathways by responding to the levels of heme and other metabolites. Recent research has highlighted its role in modulating energy homeostasis, lipid metabolism, and immune responses, suggesting that it may serve as a therapeutic target for metabolic diseases. Additionally, NR1D1 has been implicated in cancer, as its dysregulation can affect tumor growth and progression. The recombinant expression of NR1D1 protein is essential for further functional studies to elucidate its mechanisms of action, and for screening potential pharmacological agents that can modulate its activity. Understanding the molecular pathways governed by NR1D1 not only sheds light on circadian biology but also opens avenues for the development of novel treatments for related metabolic and inflammatory conditions.
