Analytical Data
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Gene name
PCr
- Application
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Alternative Names
PCr；Coiled-coil domain-containing Protein 62
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P9F0
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Expression Region
1-684aa
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AA Sequence
MNPPAAFLAGRQNIGSEVEISTIEKQRKELQLLIGELKDRDKELNDMVAVHQQQLLSWEEDRQKVLTLEERCSKLEGELHKRTEIIRSLTKKVKALESNQMECQTALQKTQLQLQEMAQKATHSSLLSEDLEARNETLSNTLVELSAQVGQLQAREQALTTMIKLKDKDIIEAVNHIADCSGKFKMLEHALRDAKMAETCIVKEKQDYKQKLKALKIEVNKLKEDLNEKTTENNEQREEIIRLKQEKSCLHDELLFTVEREKRKDELLNIAKSKQERTNSELHNLRQIYVKQQSDLQFLNFNVENSQELIQMYDSKMEESKALDSSRDMCLSDLENNHPKVDIKREKNQKSLFKDQKFEAMLVQQNRSDKSSCDECKEKKQQIDTVFGEKSVITLSSIFTKDLVEKHNLPWSLGGKTQIEPENKITLCKIHTKSPKCHGTGVQNEGKQPSETPTLSDEKQWHDVSVYLGLTNCPSSKHPEKLDVECQDQMERSEISCCQKNEACLGESGMCDSKCCHPSNFIIEAPGHMSDVEWMSIFKPSKMQRIVRLKSGCTCSESICGTQHDSPASELIAIQDSHSLGSSKSALREDETESSSNKKNSPTSLLIYKDAPAFNEKASIVLPSQDDFSPTSKLQRLLAESRQMVTDLELSTLLPISHENLTGSATNKSEVPEESAQKNTFVSY
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Molecular Weight
77.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Phosphocreatine (PCr) is a crucial energy substrate found predominantly in muscle and brain tissues, facilitating the rapid regeneration of adenosine triphosphate (ATP) during high-energy demand situations. Its supplementation has been associated with improved performance in high-intensity exercise, making it a focal point in sports science and metabolic studies. The recombinant production of PCr-related proteins, particularly creatine kinase (CK), has garnered interest due to its potential applications in biotechnology and medicine, such as in the treatment of energy metabolism disorders. CK catalyzes the conversion of creatine and ATP to PCr and adenosine diphosphate (ADP), playing a vital role in cellular energy homeostasis. Beyond exercise physiology, the study of PCr and its associated proteins has implications for neuroprotective strategies, as these proteins may help mitigate damage in conditions like ischemia or neurodegenerative diseases. Advances in recombinant DNA technology have enabled the efficient production of these proteins, allowing for detailed biochemical studies and therapeutic exploration. Researchers aim to elucidate the structure-function relationships of CK and other related enzymes, heightening our understanding of energy metabolism and pointing toward novel interventions for enhancing muscular and cognitive functions. As our comprehension of PCr's biochemical pathways deepens, the potential for developing innovative treatments and performance enhancers continues to expand, marking an exciting frontier in both sports and medical research.
