Analytical Data
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基因名
PCr
- Application
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别名
PCr；Coiled-coil domain-containing Protein 62
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6P9F0
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表达区间
1-684aa
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氨基酸序列
MNPPAAFLAGRQNIGSEVEISTIEKQRKELQLLIGELKDRDKELNDMVAVHQQQLLSWEEDRQKVLTLEERCSKLEGELHKRTEIIRSLTKKVKALESNQMECQTALQKTQLQLQEMAQKATHSSLLSEDLEARNETLSNTLVELSAQVGQLQAREQALTTMIKLKDKDIIEAVNHIADCSGKFKMLEHALRDAKMAETCIVKEKQDYKQKLKALKIEVNKLKEDLNEKTTENNEQREEIIRLKQEKSCLHDELLFTVEREKRKDELLNIAKSKQERTNSELHNLRQIYVKQQSDLQFLNFNVENSQELIQMYDSKMEESKALDSSRDMCLSDLENNHPKVDIKREKNQKSLFKDQKFEAMLVQQNRSDKSSCDECKEKKQQIDTVFGEKSVITLSSIFTKDLVEKHNLPWSLGGKTQIEPENKITLCKIHTKSPKCHGTGVQNEGKQPSETPTLSDEKQWHDVSVYLGLTNCPSSKHPEKLDVECQDQMERSEISCCQKNEACLGESGMCDSKCCHPSNFIIEAPGHMSDVEWMSIFKPSKMQRIVRLKSGCTCSESICGTQHDSPASELIAIQDSHSLGSSKSALREDETESSSNKKNSPTSLLIYKDAPAFNEKASIVLPSQDDFSPTSKLQRLLAESRQMVTDLELSTLLPISHENLTGSATNKSEVPEESAQKNTFVSY
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分子量
77.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Phosphocreatine (PCr) is a crucial energy substrate found predominantly in muscle and brain tissues, facilitating the rapid regeneration of adenosine triphosphate (ATP) during high-energy demand situations. Its supplementation has been associated with improved performance in high-intensity exercise, making it a focal point in sports science and metabolic studies. The recombinant production of PCr-related proteins, particularly creatine kinase (CK), has garnered interest due to its potential applications in biotechnology and medicine, such as in the treatment of energy metabolism disorders. CK catalyzes the conversion of creatine and ATP to PCr and adenosine diphosphate (ADP), playing a vital role in cellular energy homeostasis. Beyond exercise physiology, the study of PCr and its associated proteins has implications for neuroprotective strategies, as these proteins may help mitigate damage in conditions like ischemia or neurodegenerative diseases. Advances in recombinant DNA technology have enabled the efficient production of these proteins, allowing for detailed biochemical studies and therapeutic exploration. Researchers aim to elucidate the structure-function relationships of CK and other related enzymes, heightening our understanding of energy metabolism and pointing toward novel interventions for enhancing muscular and cognitive functions. As our comprehension of PCr's biochemical pathways deepens, the potential for developing innovative treatments and performance enhancers continues to expand, marking an exciting frontier in both sports and medical research.
