Analytical Data
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Gene name
RIOK3
- Application
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Alternative Names
RIOK3；SUDD；Serine/threonine-Protein kinase RIO3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14730
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Expression Region
1-519aa
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AA Sequence
MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE
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Molecular Weight
62.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RIOK3, a member of the RIO kinase family, has garnered attention due to its essential role in cellular processes such as ribosome biogenesis and cell proliferation. Emerging evidence suggests that RIOK3 plays a critical function in the regulation of the cell cycle, particularly in the G1 to S phase transition, which is crucial for DNA replication and cell division. Dysregulation of RIOK3 has been associated with various cancers, highlighting its potential as a biomarker and therapeutic target. Studies have shown that RIOK3 interacts with several key cellular pathways, influencing not only cell growth but also stress responses and apoptosis. Its distinct kinase activity further qualifies it as a promising candidate for drug development, aimed at modulating its function in tumorigenesis. Recent research has focused on elucidating the molecular mechanisms governing RIOK3's activity, including post-translational modifications and interaction partners. This makes RIOK3 a pivotal player in understanding cellular mechanics and developing new therapeutic strategies in oncology. Given its implications in disease biology, ongoing studies aim to characterize RIOK3’s structure-function relationships and its potential interactions within various signaling networks, ultimately advancing our comprehension of target-based cancer treatments.
