Analytical Data
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Gene name
MOBKL2B
- Application
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Alternative Names
MOB3B; C9orf35; MOBKL2B; MOB kinase activator 3B; Mob1 homolog 2b; Mps one binder kinase activator-like 2B; MOB kinase activator-like 2B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86TA1
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Expression Region
1-216 aa
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AA Sequence
MSIALKQVFN KDKTFRPKRK FEPGTQRFEL HKRAQASLNS GVDLKAAVQL PSGEDQNDWV AVHVVDFFNR INLIYGTICE FCTERTCPVM SGGPKYEYRW QDDLKYKKPT ALPAPQYMNL LMDWIEVQIN NEEIFPTCVG VPFPKNFLQI CKKILCRLFR VFVHVYIHHF DRVIVMGAEA HVNTCYKHFY YFVTEMNLID RKELEPLKEM TSRMCH
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Molecular Weight
25.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MOBKL2B, a member of the MOB (Mps One Binder) protein family, plays a crucial role in various cellular processes, including cell cycle regulation, mitotic progression, and signal transduction. Its involvement in the Hippo pathway, which is pivotal for controlling cell growth and organ size, has garnered significant attention in recent years. Dysregulation of MOBKL2B has been associated with several cancers, making it a potential target for therapeutic interventions. Studies have shown that MOBKL2B interacts with various kinases, particularly LATS1/2, influencing the phosphorylation and activation state of downstream effectors like YAP/TAZ, which are key modulators of cell proliferation and apoptosis. Given its importance in tumorigenesis and cellular homeostasis, the production and characterization of recombinant MOBKL2B protein have become an essential step in elucidating its structure-function relationships. Researchers aim to explore its biochemical properties, functional roles, and therapeutic potentials by using techniques like X-ray crystallography and NMR spectroscopy. Additionally, understanding how MOBKL2B regulates key cellular pathways may pave the way for novel cancer treatment strategies and contribute to the development of biomarker platforms for early cancer detection and prognosis. Therefore, the study of MOBKL2B recombinant protein not only enhances our knowledge of fundamental biological processes but also opens avenues for innovative cancer therapies.
