Analytical Data
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基因名
POP4
- Application
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别名
POP4;RPP29;Ribonuclease P Protein subunit p29
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95707
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表达区间
1-220aa
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氨基酸序列
MKSVIYHALSQKEANDSDVQPSGAQRAEAFVRAFLKRSTPRMSPQAREDQLQRKAVVLEYFTRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLHELWKQYIRDLCSGLKPDTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL
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分子量
41.4kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POP4 is a crucial component of the exosome complex, which plays a vital role in RNA processing and degradation in eukaryotic cells. The exosome is composed of a core complex with multiple subunits, and POP4, as one of these subunits, is essential for maintaining the structural integrity and functional efficiency of the exosome. Research into POP4 has garnered interest due to its involvement in various cellular processes, including the regulation of gene expression and the maintenance of RNA homeostasis. Aberrations in exosomal functions, including those involving POP4, have been linked to several diseases, including cancer and neurodegenerative disorders, making the understanding of its structure and function critical. Furthermore, POP4’s role in RNA surveillance mechanisms highlights its importance in cellular defense against defective or unwanted RNA molecules, which could otherwise lead to detrimental cellular consequences. Investigating the biochemical properties and interaction networks of POP4 can provide insights into the molecular mechanisms of exosome-related pathways and may reveal potential therapeutic targets. Consequently, studies focusing on the expression, purification, and functional characterization of POP4 recombinantly have been essential in elucidating its role in RNA metabolism and its broader implications in health and disease.
