Analytical Data
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Gene name
EphB4
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简介
Ephb4 Protein, a receptor tyrosine kinase, plays a critical role in angiogenesis and development. Dysregulation of Ephb4 Protein has been associated with disorders like cancer and cardiovascular diseases. Targeting Ephb4 Protein may provide potential therapeutic interventions in these conditions by modulating angiogenesis, inhibiting tumor growth, and managing cardiovascular complications. EphB4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB4 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Immobilized Recombinant Mouse EphB4 is immobilized at 2 µg/mL(100 µL/well) can bind Mouse Ephrin-B2 (HY-P73017). The ED50 for this effect is 1.803 ng/mL. Immobilized Recombinant Mouse EphB4 is immobilized at 2 µg/mL (100 µL/well) can bind Mouse Ephrin-B2 (HY-P73017). The ED50 for this effect is 1.803 ng/mL.
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Alternative Names
Ephrin type-B receptor 4; EPHB4; HTK; MYK1; TYRO11
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P54761
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Expression Region
L16-A539
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AA Sequence
LEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPETVPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLA
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Protein Length
Extracellular Domain
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Molecular Weight
75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EphB4, a member of the Eph receptor family, plays a critical role in various biological processes, including cell signaling, tissue development, and angiogenesis. It primarily interacts with ephrin-B ligands, facilitating bidirectional signaling that is essential for processes such as neuronal development and the regulation of vascular systems. Recent studies have highlighted the involvement of EphB4 in tumor biology, where abnormal expression or activation can contribute to tumor progression, metastasis, and the modulation of the immune response. Consequently, EphB4 has emerged as a potential therapeutic target in cancer treatment, prompting researchers to investigate its role in tumor microenvironments and the mechanisms underlying its function. The development of EphB4 recombinant proteins has provided valuable tools for understanding its signaling pathways and interactions. These proteins can be utilized in various applications, including cell adhesion assays, receptor-ligand interaction studies, and functional assays in cellular models. Moreover, the study of EphB4 recombinant proteins aims to elucidate their potential in developing targeted therapies, thereby enhancing our understanding of their role in pathology and paving the way for innovative therapeutic strategies in oncology and regenerative medicine.
