Analytical Data
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Gene name
EphB3
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简介
The EphB3 protein is a receptor tyrosine kinase that performs bidirectional signaling with the transmembrane ephrin B ligand. It participates in forward signaling and reverse signaling, sharing functionality with EPHB2. EphB3 Protein, Human (HEK293, His) is the recombinant human-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1
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Species
Human
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54753
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Expression Region
G34-L559
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Protein Length
Extracellular Domain
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Molecular Weight
65-68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EphB3 is a member of the Eph receptor tyrosine kinase family, which plays crucial roles in various cellular processes, including cell adhesion, migration, and differentiation. These receptors are primarily involved in the neural and vascular systems, influencing developmental processes and tissue organization. Dysregulation of EphB3 signaling has been implicated in several pathological conditions, including cancer, where it has been associated with tumor progression and metastasis. The study of EphB3 recombinant proteins is vital for understanding the structural and functional mechanisms underlying its activity. Researchers utilize recombinant protein techniques to produce EphB3 in a controlled environment, allowing for detailed analysis of its signaling pathways and interactions with ligands, particularly ephrins. This has significant implications for therapeutic applications, as targeted modulation of EphB3 signaling may provide novel strategies for cancer treatment and regenerative medicine. By generating EphB3 recombinant proteins, scientists can investigate its role in cellular contexts and explore potential interventions that could mitigate the effects of its dysregulation in disease states. Consequently, the ongoing research on EphB3 recombinant proteins not only expands our understanding of Eph receptor biology but also paves the way for innovative approaches to tackle various diseases linked to its aberrant functions.
