Analytical Data
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基因名
MED22
- Application
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别名
MED22; SURF5Mediator of RNA polymerase II transcription subunit 22; Mediator complex subunit 22; Surfeit locus protein 5; Surf-5
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15528
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表达区间
1-140aa
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氨基酸序列
MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSRYK
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分子量
42.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MED22, a subunit of the Mediation complex, plays a crucial role in the regulation of gene expression by serving as a bridge between transcription factors and the RNA polymerase II machinery. As a component of the Mediator complex, which is essential for transcriptional regulation of protein-coding genes, MED22 is involved in various cellular processes, including cell differentiation, proliferation, and response to external stimuli. Recent studies have highlighted MED22's involvement in several biological pathways, linking it to various diseases, including cancer and neurodegenerative disorders. Given its pivotal role in gene regulation, researchers are increasingly interested in understanding the molecular mechanisms underlying MED22 function. This includes the elucidation of its structural properties, interaction with other Mediator components, and the identification of its target genes. Furthermore, the elucidation of MED22's role in specific pathways may provide insights into its potential as a therapeutic target. The production of recombinant MED22 protein is essential for such studies as it allows for in-depth biochemical analyses and functional assays, aiding in the characterization of its role in transcription regulation. Overall, the research on MED22 not only enhances our understanding of transcriptional control but also opens avenues for therapeutic approaches in diseases associated with its dysfunction.












