Analytical Data
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基因名
ALOX12B
- Application
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别名
ALOX12B；Arachidonate 12-lipoxygenase. 12R-type
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75342
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表达区间
1-701aa
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氨基酸序列
MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAV GQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFP AYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFL PGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNL RYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVV SEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGE GTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGK MMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLL ETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGG LSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRD DSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRES SGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMR NPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPD IHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSIS I
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分子量
80.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALOX12B, a member of the lipoxygenase family, plays a crucial role in the metabolism of arachidonic acid and the production of bioactive lipid mediators. Its significance has been highlighted in various biological processes, including inflammation, skin development, and the response to oxidative stress. Mutations in the ALOX12B gene have been linked to congenital disorders such as harlequin ichthyosis, a severe skin disorder characterized by thickened, scaly skin. The recombinant expression of ALOX12B protein allows researchers to elucidate its enzymatic mechanisms and interactions with other cellular pathways. By studying this protein in a controlled setting, scientists aim to gain insights into its role in lipid signaling and its potential therapeutic applications in skin-related diseases and inflammatory conditions. The generation of ALOX12B recombinant protein not only enhances our understanding of its biological functions but also paves the way for the development of novel treatments targeting aberrant lipid metabolism and associated pathologies.
