Analytical Data
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Gene name
PAPSS1
- Application
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Alternative Names
PAPSS1;ATPSK1;PAPSS;Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43252
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Expression Region
22-624aa
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AA Sequence
MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMA LEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFA DAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVK GLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQ ERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLA EGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLD GCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVME QGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLR NPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQ HAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVG RDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKR MDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSL EKA
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Molecular Weight
95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAPSS1 (3'-phosphoadenosine-5'-phosphate sec-alpha-sulfotransferase 1) is an essential enzyme involved in the biosynthesis of sulfate metabolites, playing a critical role in the sulfate activation pathway that synthesizes 3'-phosphoadenosine 5'-phosphate (PAP) and subsequently facilitates the transfer of sulfate groups to various substrates. The study of PAPSS1 is particularly significant due to its involvement in the regulation of sulfate homeostasis, which is crucial for numerous biological processes, including drug metabolism, hormone regulation, and the synthesis of glycosaminoglycans. Dysregulation of PAPSS1 has been implicated in various pathological conditions, including certain cancers and developmental disorders, emphasizing the need for a thorough understanding of its function and mechanisms. Additionally, the recombinant expression of PAPSS1 offers the potential for in-depth biochemical studies and the development of therapeutic strategies targeting sulfate-related dysfunctions. Current research focuses on optimizing the production of PAPSS1 in heterologous systems to facilitate studies on its enzymatic activity, structural biology, and interactions with other biomolecules. Understanding the precise role of PAPSS1 at the molecular level could pave the way for novel approaches in treating diseases linked to sulfation imbalances, making it a compelling target for further investigation in pharmaceutical and biomedical research.
