Analytical Data
-
Gene name
MAP3K2
- Application
-
Alternative Names
M3K2_HUMAN; Map3k2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2b; Mitogen activated protein kinase kinase kinase 2 ; Mitogen-activated protein kinase kinase kinase 2
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9Y2U5
-
Expression Region
1-619aa
-
AA Sequence
MDDQQALNSI MQDLAVLHKA SRPALSLQET RKAKSSSPKK QNDVRVKFEH RGEKRILQFP RPVKLEDLRS KAKIAFGQSM DLHYTNNELV IPLTTQDDLD KAVELLDRSI HMKSLKILLV INGSTQATNL EPLPSLEDLD NTVFGAERKK RLSIIGPTSR DRSSPPPGYI PDELHQVARN GSFTSINSEG EFIPESMDQM LDPLSLSSPE NSGSGSCPSL DSPLDGESYP KSRMPRAQSY PDNHQEFSDY DNPIFEKFGK GGTYPRRYHV SYHHQEYNDG RKTFPRARRT QGTSLRSPVS FSPTDHSLST SSGSSIFTPE YDDSRIRRRG SDIDNPTLTV MDISPPSRSP RAPTNWRLGK LLGQGAFGRV YLCYDVDTGR ELAVKQVQFD PDSPETSKEV NALECEIQLL KNLLHERIVQ YYGCLRDPQE KTLSIFMEYM PGGSIKDQLK AYGALTENVT RKYTRQILEG VHYLHSNMIV HRDIKGANIL RDSTGNVKLG DFGASKRLQT ICLSGTGMKS VTGTPYWMSP EVISGEGYGR KADIWSVACT VVEMLTEKPP WAEFEAMAAI FKIATQPTNP KLPPHVSDYT RDFLKRIFVE AKLRPSADEL LRHMFVHYH
-
Molecular Weight
69,7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAP3K2, also known as Mitogen-Activated Protein Kinase Kinase Kinase 2, is a crucial player in the MAPK signaling pathways, which are fundamental for regulating various cellular processes, including growth, differentiation, and response to stress. This protein acts as an upstream regulator that activates the MAPK cascade, impacting various downstream effectors involved in essential cellular functions. The study of MAP3K2 is particularly relevant in the context of cancer, as aberrations in its signaling pathways have been linked to tumorigenesis and cancer progression. Furthermore, MAP3K2 has been implicated in a range of other diseases, including cardiovascular and neurodegenerative disorders. The recombinant expression of MAP3K2 allows for a detailed examination of its biochemical properties, interaction partners, and functional roles in signaling pathways. By utilizing recombinant technology, researchers can produce purified MAP3K2 protein in sufficient quantities for various assays, including enzymatic activity studies, protein-protein interaction analyses, and structure-function investigations. Understanding the functional mechanisms of MAP3K2 through recombinant protein studies can provide insights into its biological roles and help identify potential therapeutic targets for diseases associated with dysregulated MAPK signaling. Given its central role in cellular signaling, MAP3K2 continues to be a focal point for research aimed at unraveling the complexities of MAPK pathways and their implications in health and disease.
