Analytical Data
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Gene name
PCDH19
- Application
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Alternative Names
PCDH19；KIAA1313；Protocadherin-19
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TAB3
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Expression Region
22-678aa
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AA Sequence
LINLKYSVEEEQRAGTVIANVAKDAREAGFALDPRQASAFRVVSNSAPHLVDINPSSGLLVTKQKIDRDLLCRQSPKCIISLEVMSSSMEICVIKVEIKDLNDNAPSFPAAQIELEISEAASPGTRIPLDSAYDPDSGSFGVQTYELTPNELFGLEIKTRGDGSRFAELVVEKSLDRETQSHYSFRITALDGGDPPRLGTVGLSIKVTDSNDNNPVFSESTYAVSVPENSPPNTPVIRLNASDPDEGTNGQVVYSFYGYVNDRTRELFQIDPHSGLVTVTGALDYEEGHVYELDVQAKDLGPNSIPAHCKVTVSVLDTNDNPPVINLLSVNSELVEVSESAPPGYVIALVRVSDRDSGLNGRVQCRLLGNVPFRLQEYESFSTILVDGRLDREQHDQYNLTIQARDGGVPMLQSAKSFTVLITDENDNHPHFSKPYYQVIVQENNTPGAYLLSVSARDPDLGLNGSVSYQIVPSQVRDMPVFTYVSINPNSGDIYALRSFNHEQTKAFEFKVLAKDGGLPSLQSNATVRVIILDVNDNTPVITAPPLINGTAEVYIPRNSGIGYLVTVVKAEDYDEGENGRVTYDMTEGDRGFFEIDQVNGEVRTTRTFGESSKSSYELIVVAHDHGKTSLSASALVLIYLSPALDAQESMGSVNLS
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Molecular Weight
100.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PCDH19 (Protocadherin 19) is a member of the cadherin superfamily and plays a critical role in regulating cell-cell interactions within the developing nervous system. Mutations in the PCDH19 gene have been implicated in a rare X-linked neurodevelopmental disorder known as PCDH19-related epilepsy, characterized by early-onset seizures and cognitive impairment, primarily affecting females due to X-inactivation patterns. Research into PCDH19 has gained momentum as understanding its structure and function could provide insights into underlying pathogenic mechanisms and potential therapeutic targets. The production of recombinant PCDH19 protein is essential for investigating its molecular characteristics, including binding affinities, structural properties, and interaction with other proteins. This study aims to generate and characterize PCDH19 recombinant protein, utilizing various expression systems to ensure proper folding and functionality. By examining its role in neuronal development and synaptic plasticity, this research contributes to a broader understanding of PCDH19's involvement in epilepsy and neurodevelopmental disorders, paving the way for future therapeutic strategies. Additionally, insights gained from PCDH19 protein interactions may benefit the fields of neurobiology and genetics, offering potential advancements in precision medicine approaches for patients affected by PCDH19-related disorders. Through this work, we hope to elucidate the multifaceted roles of PCDH19 in neuronal function and pathology, providing a foundational step toward the development of targeted interventions for affected individuals.
