Analytical Data
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Gene name
EphA8
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简介
The EPHA8 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It participates in forward and reverse signaling pathways and is phosphorylated by ligands such as EFNA2, EFNA3, and EFNA5. EphA8 Protein, Human (HEK293, His) is the recombinant human-derived EphA8 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
rHuEphrin type-A receptor 8/EphA8, His; EEK; EK3; HEK3; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH receptor A8; EphA8; EPH-like kinase 3; ephrin type-A receptor 8; Hek3
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29322-1
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Expression Region
E31-T542
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AA Sequence
EVNLLDTSTIHGDWGWLTYPAHGWDSINEVDESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVACELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVNLISSVNGTSVTLEWAPPLDPGGRSDITYNAVCRRCPWALSRCEACGSGTRFVPQQTSLVQASLLVANLLAHMNYSFWIEAVNGVSDLSPEPRRAAVVNITTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEKDKEMQSYSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVETGKPRPRYDTRT
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Protein Length
Extracellular Domain
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Molecular Weight
65-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EphA8 is a member of the Eph receptor family, which is the largest group of receptor tyrosine kinases in the human genome. This family is known for its critical roles in developmental processes, particularly in the formation of neural networks and the regulation of cell adhesion and migration. EphA8 is primarily involved in mediating cell-cell interactions during embryonic development and has been implicated in various physiological and pathological processes, including neurogenesis, immune responses, and tumorigenesis. Recent studies have suggested that EphA8 plays a significant role in cancer progression, particularly in certain types of tumors, making it a potential target for therapeutic interventions. The investigation of EphA8 recombinant protein is crucial for understanding its biological functions and mechanisms of action at a molecular level. Producing high-quality recombinant EphA8 protein allows for functional assays, structural studies, and drug development efforts, contributing to the broader understanding of its role in health and disease. Consequently, the study of EphA8 is important not just for basic research, but also for its implications in developing novel therapeutic strategies for diseases linked to its dysregulation.
