Analytical Data
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基因名
NDUFS3
- Application
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别名
NDUFS3;NADH dehydrogenase [ubiquinone] iron-sulfur Protein 3. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75489
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表达区间
37-264aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMESAGADTRPTVRPRNDVAHKQLSAFGEYV AEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLT AVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANW YEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDE VKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK
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分子量
29 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFS3, a vital subunit of the mitochondrial respiratory chain complex I, plays a crucial role in cellular energy production via oxidative phosphorylation. Mutations in the NDUFS3 gene are associated with a range of mitochondrial disorders, leading to severe clinical manifestations including neurological deficits, muscle weakness, and multisystem complications. Understanding the structure and function of NDUFS3 is essential for elucidating the mechanisms underlying complex I's role in energy metabolism and its implications in various diseases. Recent studies have shown that NDUFS3 interacts with other subunits and cofactors, which are crucial for the assembly and stability of complex I. The recombinant production of NDUFS3 allows for detailed biochemical analyses, enabling researchers to investigate its functional properties, interactions, and regulatory mechanisms. Furthermore, studying NDUFS3 can provide insights into potential therapeutic strategies for treating mitochondrial dysfunctions, highlighting the significance of this protein in both basic research and clinical applications.
