Analytical Data
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基因名
NDUFS2
- Application
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别名
NDUFS2;NADH dehydrogenase [ubiquinone] iron-sulfur Protein 2. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75306
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表达区间
1-463aa
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氨基酸序列
MAALRALCGFRGVAAQVLRPGAGVRLPIQPSRGVRQWQPDVEWAQQFGGA VMYPSKETAHWKPPPWNDVDPPKDTIVKNITLNFGPQHPAAHGVLRLVME LSGEMVRKCDPHIGLLHRGTEKLIEYKTYLQALPYFDRLDYVSMMCNEQA YSLAVEKLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDLGAMTPF FWLFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKN FSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQW DLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNK MPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAI EAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAI IGTQDIVFGEVDR
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分子量
79 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFS2, or NADH:ubiquinone oxidoreductase (Complex I) subunit S2, is a critical component of the mitochondrial respiratory chain, playing an essential role in cellular energy metabolism. Mutations in the NDUFS2 gene are linked to various mitochondrial diseases, particularly affecting energy production, leading to conditions such as Leigh syndrome, which manifests in neurological deficits and multi-organ dysfunction. Given the pivotal role of Complex I in oxidative phosphorylation, the study of NDUFS2 is crucial for understanding disease mechanisms and developing potential therapies targeting mitochondrial dysfunction. Research has focused on characterizing the structural and functional aspects of the NDUFS2 protein, investigating how specific mutations impair its activity and contribute to clinical phenotypes. Additionally, the generation of recombinant NDUFS2 protein allows for detailed biochemical studies, enabling scientists to explore its interactions within the mitochondrial complex and assess the efficacy of small molecules aimed at restoring its function. As mitochondrial diseases often result in severe clinical outcomes and limited treatment options, understanding the biology of NDUFS2 can pave the way for innovative therapeutic strategies, making it a significant focus in the field of translational medicine.
