Analytical Data
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基因名
MT-ATP6
- Application
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别名
MT-ATP6；ATP6；ATPASE6；MTATP6；ATP synthase subunit a
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00846
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表达区间
1-226
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氨基酸序列
MNENLFASFIAPTILGLPAAVLIILFPPLLIPTSKYLINNRLITTQQWLIKLTSKQMMTMHNTKGRTWSLMLVSLIIFIATTNLLGLLPHSFTPTTQLSMNLAMAIPLWAGTVIMGFRSKIKNALAHFLPQGTPTPLIPMLVIIETISLLIQPMALAVRLTANITAGHLLMHLIGSATLAMSTINLPSTLIIFTILILLTILEIAVALIQAYVFTLLVSLYLHDNT
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分子量
24.8kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MT-ATP6, a critical subunit of ATP synthase, plays a pivotal role in mitochondrial ATP production, which is essential for cellular energy metabolism. Research on MT-ATP6 has gained significant attention due to its association with various mitochondrial diseases and metabolic disorders, often resulting from mutations in the MT-ATP6 gene. These mutations can lead to impaired ATP synthesis, contributing to the pathogenesis of conditions such as Leigh syndrome, mitochondrial encephalomyopathy, and other neurodegenerative diseases. The study of MT-ATP6 recombinant proteins not only facilitates the understanding of its structure-function relationships but also aids in elucidating the mechanisms by which mutations disrupt normal mitochondrial function. Furthermore, recombinant MT-ATP6 proteins can serve as valuable tools for drug development and gene therapy approaches aimed at mitigating the effects of these mutations. Advances in techniques such as X-ray crystallography and cryo-electron microscopy have enabled researchers to visualize ATP synthase and its components in unprecedented detail, fostering insights into the conformational dynamics of the enzyme during ATP synthesis. Thus, understanding MT-ATP6 at the molecular level holds the potential to unlock new therapeutic avenues for mitochondrial diseases and improve strategies for mitochondrial gene therapy.
