Analytical Data
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Gene name
BCAR3
- Application
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Alternative Names
BCAR3；NSP2；SH2D3B；Breast cancer anti-estrogen resistance Protein 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75815
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Expression Region
1-825aa
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AA Sequence
MAAGKFASLPRNMPVNHQFPLASSMDLLSSRSPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHMGTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKELEEELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTKGRPDVAKRLSLTMGGVQAREQNLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPPQSSGVDTSPCPNSPVFRTGSEPALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPSAWLNSEANYCELNPAFATGCGRGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL
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Molecular Weight
92.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) is a significant protein in the study of breast cancer, particularly in understanding the mechanisms behind anti-estrogen resistance in hormone receptor-positive breast cancers. Research indicates that BCAR3 plays a critical role in mediating cellular responses to estrogen and is linked to the activation of various signaling pathways, including those related to cell survival and proliferation. Its expression levels are often correlated with therapeutic resistance, making it a potential biomarker for predicting treatment outcomes. The recombinant form of BCAR3 has garnered attention as scientists aim to elucidate its functional properties and interactions with other molecules involved in cancer progression. By producing BCAR3 as a recombinant protein, researchers can study its biochemical and biophysical characteristics in controlled laboratory settings. This includes exploring its role in the PI3K/Akt and MAPK signaling pathways, which are pivotal in cell metabolism, growth, and survival. Understanding BCAR3's intricate involvement in these pathways could provide insights into novel therapeutic strategies to overcome resistance in breast cancer treatment. Moreover, as researchers continue to investigate its potential as a therapeutic target, the development of BCAR3-based interventions could lead to improved treatment options for patients facing challenging and resistant forms of breast cancer. Overall, the study of BCAR3 and its recombinant form stands as a crucial area of inquiry in cancer biology, with implications for enhancing the efficacy of existing therapies and developing future treatments.
