Analytical Data
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基因名
aprA
- Application
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别名
aprA；SDH2；SDHF；Succinate dehydrogenase [ubiquinone] flavoProtein subunit. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04189
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表达区间
107-381aa
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氨基酸序列
AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
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分子量
43.7kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of aprA recombinant protein is situated within the broader context of microbial biotechnology and enzyme research. AprA, or alkaline protease A, is an important extracellular serine protease produced by certain strains of bacteria, notably from the genus Bacillus. This enzyme exhibits significant industrial potential due to its ability to function under alkaline conditions, making it suitable for applications in detergents, food processing, and pharmaceutical industries. The enzymatic activity of aprA is characterized by its stability at elevated temperatures and pH levels, which is a desirable trait for various industrial processes. Furthermore, recombinant technology allows for the production of aprA in host organisms such as Escherichia coli, facilitating the large-scale extraction and purification of this enzyme for research and application. The genetic manipulation involved in producing aprA as a recombinant protein also aids in understanding its structure-function relationship, enzymatic mechanisms, and potential modifications that can enhance its activity or stability. As researchers continue to explore the functional aspects and applications of aprA, the protein remains a focal point of interest in enzymology, synthetic biology, and the quest for sustainable industrial solutions.
