Analytical Data
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基因名
SRD5a2
- Application
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别名
SRD5a2；3-oxo-5-alpha-steroid 4-dehydrogenase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31213
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表达区间
28-65aa
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氨基酸序列
AKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPA
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分子量
30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SRD5A2 (steroid 5alpha-reductase type 2) is an enzyme vital in the metabolism of androgens, specifically in the conversion of testosterone to dihydrotestosterone (DHT), a more potent androgen. Research on SRD5A2 has gained significance due to its essential role in various physiological processes, including prostate development, hair growth, and reproductive health. Mutations in the SRD5A2 gene are linked to 5-alpha-reductase deficiency, a rare genetic condition leading to ambiguous genitalia and infertility in males. Moreover, aberrations in SRD5A2 activity are associated with several clinical conditions, such as androgenetic alopecia (male and female pattern baldness) and prostate cancer. The study of SRD5A2 recombinant proteins is crucial for understanding its structural and functional dynamics, which can inform therapeutic approaches. By producing SRD5A2 in a recombinant system, researchers can investigate its enzymatic properties, substrate interactions, and effects of inhibitors, paving the way for drug development targeting pathological conditions related to androgen metabolism. The insights gained from these studies not only enhance our knowledge of steroid hormone biology but also contribute to the development of novel treatments for disorders linked to SRD5A2 dysfunction.
