Analytical Data
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Gene name
KRT12
- Application
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Alternative Names
CK-12; Cytokeratin-12; K12; K1C12_HUMAN; Keratin 12; Keratin; Keratin; type I cytoskeletal 12; Keratin-12; KRT12; type I cytoskeletal 12
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99456
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Expression Region
1-494aa
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AA Sequence
MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSASSVGSGYGGSAFGFGASCGGGFSAASMFGSSSGFGGGSGSSMAGGLGAGYGRALGGGSFGGLGMGFGGSPGGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYLDKVRALEEANTELENKIREWYETRGTGTADASQSDYSKYYPLIEDLRNKIISASIGNAQLLLQIDNARLAAEDFRMKYENELALRQGVEADINGLRRVLDELTLTRTDLEMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM
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Molecular Weight
80.74 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KRT12, or Keratin 12, is a type of keratin protein predominantly expressed in the corneal epithelium, playing a critical role in maintaining the structural integrity and transparency of the cornea. Understanding the functions and regulatory mechanisms of KRT12 is essential for insights into various ocular diseases, including keratoconus and corneal dystrophies, where abnormal keratin expression can lead to severe vision impairments. Research on recombinant KRT12 proteins has become increasingly relevant as it allows scientists to study the protein's properties in a controlled laboratory environment. By producing KRT12 in a recombinant form, researchers can explore its molecular interactions, post-translational modifications, and functional responses to physiological conditions. Additionally, recombinant KRT12 can be used in therapeutic applications, such as tissue engineering and regenerative medicine, aiming to develop new strategies for corneal repair and restoration of vision. The growing body of research surrounding KRT12 not only enhances our understanding of corneal biology but also opens pathways for innovative treatments targeting corneal health and disease management. Thus, the study of KRT12 recombinant proteins is pivotal for developing therapeutic approaches to mitigate corneal disorders and improve ocular health outcomes.
