Analytical Data
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Gene name
HNF4a
- Application
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Alternative Names
HNF4a；HNF4；NR2A1；TCF14；Hepatocyte nuclear factor 4-alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41235
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Expression Region
1-474aa
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AA Sequence
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
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Molecular Weight
59.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HNF4α (Hepatocyte Nuclear Factor 4 Alpha) is a transcription factor primarily expressed in the liver, pancreas, and intestine, playing a crucial role in regulating metabolic processes, developmental pathways, and the maintenance of organ function. Mutations or dysregulation of HNF4α have been implicated in various metabolic disorders, including diabetes and fatty liver disease. Due to its significant role in metabolic regulation, research on HNF4α has garnered attention in both basic and applied sciences, particularly for its potential therapeutic applications. Recombinant HNF4α proteins are generated to study their structure-function relationships, elucidate their mechanisms of action, and explore their interactions with other biomolecules. These studies can provide insights into the molecular pathways that HNF4α regulates and aid in the identification of novel drug targets for metabolic diseases. Furthermore, understanding HNF4α's role in transcriptional regulation can enhance our knowledge of liver function and contribute to the development of strategies for metabolic syndrome and liver-associated disorders. Consequently, the production and characterization of recombinant HNF4α protein not only advance academic research but also hold promise for translational applications in medicine.
