Analytical Data
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Gene name
KIF15
- Application
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Alternative Names
KIF15; KLP2; KNSL7Kinesin-like protein KIF15; Kinesin-like protein 2; hKLP2; Kinesin-like protein 7; Serologically defined breast cancer antigen NY-BR-62
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NS87
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Expression Region
1279-1385aa
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AA Sequence
NKEMECLRMTDEVERTQTLESKAFQEKEQLRSKLEEMYEERERTSQEMEMLRKQVECLAEENGKLVGHQNLHQKIQYVVRLKKENVRLAEETEKLRAENVFLKEKKR
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Molecular Weight
37.51 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KIF15, a member of the kinesin superfamily of motor proteins, plays a crucial role in the process of mitosis, particularly in the formation and regulation of the mitotic spindle. This protein functions as a plus-end directed motor, facilitating the movement of structures such as spindle poles and chromosomes during cell division. Research has shown that KIF15 is essential for proper spindle assembly and chromosome alignment, which are critical for accurate genetic material segregation. Abnormalities in KIF15 expression or function can lead to mitotic defects, contributing to conditions such as cancer, where uncontrolled cell proliferation is a hallmark. The study of KIF15 recombinant protein has gained traction as a means to better understand its structural and functional properties, including its interactions with other spindle components and the mechanisms that govern its motor activity. By employing various biochemical and biophysical techniques, researchers aim to elucidate how KIF15 contributes to the dynamic processes of cell division and how it may serve as a potential therapeutic target in the treatment of cancer and other diseases associated with mitotic errors. This exploration not only advances our fundamental knowledge of cellular mechanics but also opens avenues for the development of innovative therapeutic strategies that could mitigate the consequences of abnormal cell division.
