Analytical Data
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Gene name
UGT1A8
- Application
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Alternative Names
UGT1A8；GNT1；UGT1；UDP-glucuronosyltransferase 1A8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HAW9
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Expression Region
26-530aa
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AA Sequence
GKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLGKSLNCTVKTYSTSYTLEDLDREFMDFADAQWKAQVRSLFSLFLSSSNGFFNLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDACGLIVAKYFSLPSVVFARGIACHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLFCQYFSKNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH
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Molecular Weight
59.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UGT1A8 (Uridine 5'-diphospho-glucuronosyltransferase 1A8) is an enzyme that plays a crucial role in the process of drug metabolism and detoxification in the human body. It is part of the uridine diphospho-glucuronosyltransferase (UGT) family, which is responsible for the conjugation of various endogenous and exogenous compounds with glucuronic acid. This process, known as glucuronidation, enhances the solubility and excretion of these substances, thereby influencing their pharmacological activity and toxicity. Research on UGT1A8 has gained significance due to its involvement in the metabolism of a variety of therapeutic agents and environmental toxins, contributing to individual variations in drug response and susceptibility to adverse effects. Furthermore, UGT1A8 is implicated in the metabolism of certain carcinogens, making its study relevant in cancer research. Given the clinical importance of UGT1A8 and its potential impact on drug development, efforts have been made to understand its structure, function, and regulatory mechanisms. Recombinant UGT1A8 protein is thus generated for in vitro studies, enabling researchers to investigate its enzymatic properties, substrate specificity, and interactions with various drugs. This knowledge not only advances the understanding of metabolic pathways but also aids in predicting drug-drug interactions and optimizing therapeutic regimens, which are critical for personalized medicine approaches. Overall, the study of UGT1A8 and its recombinant form is pivotal for improving our grasp of pharmacogenomics and enhancing drug safety and efficacy.
