Analytical Data
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基因名
YTHDF2
- Application
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别名
YTHDF2；HGRG8；YTH domain-containing family Protein 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y5A9
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表达区间
2-579aa
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氨基酸序列
SASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK
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分子量
68.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
YTHDF2 is a member of the YTH domain family of proteins, which are known to recognize and bind to N6-methyladenosine (m6A) modifications on RNA molecules. This modification is the most prevalent internal mRNA modification and plays a crucial role in regulating various aspects of RNA metabolism, including RNA stability, splicing, transport, and translation. Research has shown that YTHDF2 is primarily involved in the degradation of m6A-containing mRNAs, thereby influencing gene expression and cellular processes. The dysregulation of YTHDF2 has been linked to several diseases, including cancers, emphasizing the importance of understanding its functional mechanisms. Furthermore, studying YTHDF2 as a recombinant protein provides valuable insights into its structural properties and binding affinities, facilitating the exploration of novel therapeutic strategies targeting m6A pathways. The generation and characterization of recombinant YTHDF2 protein allow researchers to conduct in vitro assays to investigate its interaction with RNA and other regulatory proteins, shedding light on its role in post-transcriptional gene regulation. Overall, the study of YTHDF2 and its recombinant protein form is pivotal in elucidating the complex regulatory networks of m6A modifications and their implications in health and disease.
